Resources

The DCI offers cryo-electron microscopy and cryo-electron tomography studies as a service for a fee.

This includes among others the following:

  • Consulting in structural analysis possibilities to answer a research question
  • Sample preparation and optimization for cryo-EM investigations
  • Data collection by SPA or cryo-ET methods
  • Image processing and 3D structure reconstruction
  • Building of atomic models
  • Assistance in the interpretation of the results

Microscopes

Image Processing Computer Software employed by the DCI
The DCI uses several workstations to process data on the fly during data collection. This will utilize the FOCUS software package, as well as several other software packages.

In addition, the DCI is equipped and has the expertise to provide the entire pipeline of data processing, from motion correction, CTF correction, single particle cryo-EM processing to 3D visualization and atomic model building.
300kV
  • Titan Krios #1
    E-CFEG, SelectrisX, Falcon4i
  • Titan Krios #2
    E-CFEG, Falcon4i
  • Titan Krios #3
    E-CFEG, SelectrisX, Falcon4i
200kV
  • Glacios
    X-FEG and Falcon4

Sample preparation BSL 1

In addition, the DCI is equipped and has the expertise to provide the entire pipeline of data processing, from motion correction, CTF correction, single particle cryo-EM processing to 3D visualization and atomic model building.
  • Vitrobot Mark IV
  • Chameleon
  • Leica EM GP2

Sample preparation BSL 2

  • Vitrobot Mark IV
  • Leica EM GP2

Software

Image Processing Computer Software employed by the DCI
The DCI uses several workstations to process data on the fly during data collection. This will utilize the FOCUS software package, as well as several other software packages.

In addition, the DCI is equipped and has the expertise to provide the entire pipeline of data processing, from motion correction, CTF correction, single particle cryo-EM processing to 3D visualization and atomic model building.
  • Serial-EM
  • TOMO5-TFS
  • EPU-TFS
  • SBGrid

Computing Hardware

The DCI uses several workstations to process data on the fly during data collection. This will utilize the FOCUS software package, as well as several other software packages.
  • Data Storage

Fees

Invoices
Acknowledgements
Co-authorships
Policies
Services agreement

Services of the DCI are always billed. In addition to the fees, we kindly request that you acknowledge the DCI personnel in resulting publications.

An example of a general acknowledgement: “We thank the DCI from EPFL, UNIL and UNIGE for their support”. Please inform the Head of DCI about any resulting publications or posters, as this is important for our reporting.

If a member of the DCI has contributed substantial intellectual input to your project, such as when dealing with very challenging samples, interpreting results, or building an atomic model into a 3D map, then please consider adding that person (and that person only) to the list of co-authors of a resulting publication.

The DCI-Lausanne cryo-EM center – General Policies

DCI-Lausanne Services Agreement

Members (1)Other
Swiss
Academia		Others (2)
Glacios
200kV, XFEG, Falcon 4i 		28 CHF/hr34.80 CHF/hr110 CHF /hr
Titan Krios
300kV, CFEG, Falcon 4i 		696 CHF/day835.20 CHF/day3360 CHF/day
Titan Krios
300kV CFEG, SelectrisX, Falcon 4i 		696 CHF/day835.20 CHF/day3600 CHF/day
Extra operator service
such as manual sample screening or image processing 		84 CHF/hr100.80 CHF/hr150 CHF/hr
Preparing the sample
with Vitrobot Mark IV 		12 CHF/hr14.40 CHF/hr50 CHF/hr
Preparing the sample
with Leica GP2 		12 CHF/hr14.40 CHF/hr50 CHF/hr
Preparing the sample
with Chameleon 		100 CHF/hr120 CHF/hr300 CHF/hr
Carbon grids
 15 CHF/hr15 CHF/hr15 CHF/hr
Gold/NiTi grids
 20 CHF/hr20 CHF/hr20 CHF/hr
C-clip/C-ring
 10 CHF/hr10 CHF/hr10 CHF/hr

(1)  Members are EPFL, UNI Lausanne, Uni Geneva and Uni Bern.
(2) Others are non-Swiss Academia and Industry. Prices are exclusive of tax.

  • All prices in CHF, and without guarantee. Contact us for definite prices.
  • The instrument fees above include consumables and the operator to set up and supervise the automated data collection, and some preliminary data processing will be done automatically during data collection.
  • Extensive sample optimization, manual operation of an electron microscope by DCI personnel, data processing or model building are billed as Extra Service.
  • For Others (i.e., non-Swiss Academia and industry clients), no additional overhead applies, but VAT has to be added where applicable.