Resources

The DCI offers cryo-electron microscopy and cryo-electron tomography studies as a service for a fee.

This includes among others the following:

  • Consulting in structural analysis possibilities to answer a research question
  • Sample preparation and optimization for cryo-EM investigations
  • Data collection by SPA or cryo-ET methods
  • Image processing and 3D structure reconstruction
  • Building of atomic models
  • Assistance in the interpretation of the results

Microscopes

Image Processing Computer Software employed by the DCI
The DCI uses several workstations to process data on the fly during data collection. This will utilize the FOCUS software package, as well as several other software packages.

In addition, the DCI is equipped and has the expertise to provide the entire pipeline of data processing, from motion correction, CTF correction, single particle cryo-EM processing to 3D visualization and atomic model building.
300kV
  • Titan Krios #1
    E-CFEG, SelectrisX, Falcon4i
  • Titan Krios #2
    E-CFEG, Falcon4i
  • Titan Krios #3
    E-CFEG, SelectrisX, Falcon4i
200kV
  • Glacios
    X-FEG and Falcon4

Sample preparation BSL 1

In addition, the DCI is equipped and has the expertise to provide the entire pipeline of data processing, from motion correction, CTF correction, single particle cryo-EM processing to 3D visualization and atomic model building.
  • Vitrobot Mark IV
  • Chameleon
  • Leica EM GP2

Sample preparation BSL 2

  • Vitrobot Mark IV
  • Leica EM GP2

Software

Image Processing Computer Software employed by the DCI
The DCI uses several workstations to process data on the fly during data collection. This will utilize the FOCUS software package, as well as several other software packages.

In addition, the DCI is equipped and has the expertise to provide the entire pipeline of data processing, from motion correction, CTF correction, single particle cryo-EM processing to 3D visualization and atomic model building.
  • Serial-EM
  • TOMO5-TFS
  • EPU-TFS
  • SBGrid

Computing Hardware

The DCI uses several workstations to process data on the fly during data collection. This will utilize the FOCUS software package, as well as several other software packages.
  • Data Storage

Fees

Invoices
Acknowledgements
Co-authorships
Policies
Services agreement

Services of the DCI are always billed. In addition to the fees, we kindly request that you acknowledge the DCI personnel in resulting publications.

An example of a general acknowledgement: “We thank the DCI from EPFL, UNIL and UNIGE for their support”. Please inform the Head of DCI about any resulting publications or posters, as this is important for our reporting.

If a member of the DCI has contributed substantial intellectual input to your project, such as when dealing with very challenging samples, interpreting results, or building an atomic model into a 3D map, then please consider adding that person (and that person only) to the list of co-authors of a resulting publication.

The DCI-Lausanne cryo-EM center – General Policies

DCI-Lausanne Services Agreement

Members (1)Other
Swiss
Academia		Others (2)
Titan #1
300kV CFEG,SelectrisX,Falcon IVi 		696/day835.20/day3600/day
Titan #2
300kV CFEG,Falcon IVi 		696/day835.20/day3360/day
Titan #3
300kV CFEG, SelectrisX, Falcon IVi 		696/day835.20/day3600/day
Glacios
200kV XFEG,FalconIVi 		29/hr
696/day		34.80/hr
835.20/day		110/hr
2640/day
Extra Service
 78/hr93.60/hr130.00/hr

(1)  Members are EPFL, UNI Lausanne, Uni Geneva and Uni Bern.
(2) Others are non-Swiss Academia and Industry. Prices are exclusive of tax.

  • All prices in CHF, and without guarantee. Contact us for definite prices.
  • The instrument fees above include consumables and the operator to set up and supervise the automated data collection, and some preliminary data processing will be done automatically during data collection.
  • Extensive sample optimization, manual operation of an electron microscope by DCI personnel, data processing or model building are billed as Extra Service.
  • For Others (i.e., non-Swiss Academia and industry clients), no additional overhead applies, but VAT has to be added where applicable.