Resources
The DCI offers cryo-electron microscopy and cryo-electron tomography studies as a service for a fee.
This includes among others the following:
- Consulting in structural analysis possibilities to answer a research question
- Sample preparation and optimization for cryo-EM investigations
- Data collection by SPA or cryo-ET methods
- Image processing and 3D structure reconstruction
- Building of atomic models
- Assistance in the interpretation of the results
Microscopes
DCI is equipped with state-of-the-art microscopes:
The DCI is currently equipped with three Titan Krios G4 fringe-free systems with Falcon 4i detectors. Two of those Titans (Krios 1 & 3) are equipped with Selectris X energy filters for mainly used for the Cryo-ET or SPA of small protein. One screening microscope, a Glacios fringe-free 200 kV system with a Falcon 4i detector has also been installed.
Software
Image Processing Computer Software employed by the DCI
The DCI uses several workstations to process data on the fly during data collection. This will utilize the FOCUS software package, as well as several other software packages.
In addition, the DCI is equipped and has the expertise to provide the entire pipeline of data processing, from motion correction, CTF correction, single particle cryo-EM processing to 3D visualization and atomic model building.
In addition, the DCI is equipped and has the expertise to provide the entire pipeline of data processing, from motion correction, CTF correction, single particle cryo-EM processing to 3D visualization and atomic model building.