Titan Krios 1 & 3:

Please find below the calibrated magnification table available for the our Krios 1 and Krios 3.

Magnification	350 kx	270 kx	215 kx	165 kx	130 kx	105 kx	81 kx
Krios 1	0.354 Å	0.452 Å	0.57 Å	0.726 Å	0.926 Å	1.2 Å	1.6 Å
Krios 3	0.355 Å	0.456 Å	0.574 Å	0.732 Å	0.934 Å	1.192 Å	1.515 Å

Other pixel size available for the our Krios 1 & 3 and Field of view associated to the magnification – For Cryo-ET usage.

Magnification	105 kx	81 kx	64 kx	53 kx	42 kx	33 kx	26 kx
Krios 1 & 3	1.2 Å	1.6 Å	2 Å	2.4 Å	3 Å	3.7 Å	4.7 Å
Field of View	500 nm	640 nm	810 nm	990 nm	1.25 µm	1.52 µm	2 µm

---

Titan Krios 2:

Please find below the calibrated magnification table available for the our Krios 2.

Magnification	250 kx	195 kx	155 kx	120 kx	96 kx	75 kx
Krios 2	0.2925 Å	0.3959 Å	0.5082 Å	0.658 Å	0.83 Å	1.063 Å

---

Glacios:

Please find below the calibrated magnification table available for the our Glacios.

Magnification	190 kx	150 kx	120 kx	92 kx
Pixel Size	0.72 Å	0.94 Å	1.2 Å	1.5 Å
C2 Aperture	20 µm	20 / 30 µm	30 µm	30 / 70 µm
Beam Size (⌀)	660 nm	660 / 1.0 µm	1.0 µm	1.09 / 2.34 µm

The microscope has currently four C2 apertures size : 20, 30, 70 and 150 µm. Any of those apertures selected for imagine should be centered. Currently the facility is using for SPA with EPU the preset with 20 or 30 µm for Grid Square, Hole/EucentricHeight and Data Acquisition, while the 150 µm aperture is usually reserved for atlas acquisition.

Reminder: Beam size on the 200 KeV TFS microscope is controled by the aperture size !

Table for parallele illumination in Nanoprobe Gun lens 3 (last calibration 2026):

Spot Size	1	2	3	4	5	6	7	8	9	10	11
C2 Lens Current (%)	NA	45.26	42.59	41	40.20	39.26	38.12	37.10	37.10	3.99	NA

---

Objective Aperture Resolution Cut-off:

Objective	100	70	50	30
Glacios	1.71 Å	2.44 Å	3.41 Å	5.5 Å
Titan	1.34 Å	1.92 Å	2.68 Å	4.5 Å