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Glacios
X-FEG and Falcon4

A TFS Glacios 200kV instrument with X-FEG and FalconIV

The 200kV Glacios with X-FEG and Falcon IV detector is a high-resolution cryo-EM instrument that is designed for resolution and throughput. Its 12-grid autoloader allows efficient loading and unloading of cryo-EM grids. The automated SerialEM and EPU software systems are used to screen grids for suitability for high-resolution analysis. The Glacios itself can determine structures are better than 2.0Å resolution in favorable cases.

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Instrument Parameters

  • Accelerating Voltage
    200 kV
  • Electron Source
    X-FEG
  • Stage Tilt Range
    ± 70°
  • C2 Apertures
    150, 100, 70 and 50 µm
  • Objective Apertures
    100, 70 and 30 µm
  • Operating Temperature
    ~80 K
  • Information Limit at 0° / ±70° tilt
    0.23 nm / 0.34 nm
  • Cs
    2.7 mm
  • Number of cartridge positions for grids
    12 total
  • Sample exchange time
    ~3 mins from autoloader cassette to column
  • Camera
    Falcon4
  • Camera pixels
    14 micrometer pixel size, 4096x4096 pixels, Electron Event Recording capable
  • Camera Performance DQE (1/2 Nyquist)
    0.70
  • Camera Performance DQE (Nyquist)
    0.28
  • Acquisition software
    SerialEM, EPU and TFS Tomography
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